Abstract: In recent years, the research and practice of traditional villages under the background of rural revitalization tend to the revitalization and utilization of cultural landscape heritage and the improvement of production and living space. Traditional villages suffer from the development dilemma of insufficient systemic protection of cultural space due to low spatial accessibility, serious population loss, insufficient industrial and economic income. Therefore, based on the scientific connotation of organic renewal, the article puts forward the theoretical framework of organic renewal of traditional village cultural space from three aspects: spatial, temporal and social. Taking the Loushang Village, Guorong Township, Shiqian County, Tongren City, Guizhou Province as an example, the article also proposed the strategies of organic renewal and revitalization of cultural space from three aspects: physical environment improvement, orderly inheritance of spiritual and culture, and positive interaction between multiple social subjects, aiming to provide a theoretical reference for the organic renewal and revitalization of cultural space in traditional villages.