Abstract: In order to better promote the rational use of urban and rural construction land, optimize national land space and help rural revitalization, this article took Lukou District of Zhuzhou City, Hunan Province as an example, an indicator system was established from two aspects as natural and human factors. The analytic hierarchy process and multi-factor weighted evaluation model were used to carry out the evaluation of the suitability of county level villages and towns based on suitability. And based on the suitability evaluation results, the conflict space was identified, and the rationality and sustainability of the regional construction space layout were evaluated. The results showed that the spatial expansion suitability of villages and towns in Lukou District were significantly different, and the spatial distribution characteristics of different levels were quite different. The areas of the most suitable, more suitable, basic suitable, less suitable and unsuitable for the spatial expansion of villages and towns in Lukou District were 132.46km², 313.41 km², 398.05 km², 325.94 km² and 160.14 km². There were obvious differences in the suitability of spatial expansion of the towns in Lukou District. The conflict space was mainly distributed in the northern urbanization development and the ecological environment fragile area in the southeast, the intensity of the spatial conflict showed a trend from low to high from south to north, and the spatial differences of conflicts in each township were obvious.