Abstract: Rural areas in developed region have the external thrust and basic advantages to take the lead in basically realizing agricultural and rural modernization and rural revitalization.The town and village layout planning takes rural space as the carrier to guide the formation of an ecological and livable town and village system, which is an important lever for rural revitalization. It is of great theoretical value and practical significance to discuss the optimization method of town and village layout planning in developed region. This paper focuses on the characteristics of rural areas in developed region and examines many problems of previous town and village layout planning. It is concluded that the optimization method of town and village layout planning for developed region in the new period should focus on three aspects: planning thinking, space optimization path, and construction management measures. Taking Jiangning District of Jiangsu Nanjing as an example, this paper puts forward the path of optimizing the layout of town and village in developed region from the aspects of strengthening the bottom line management and control and multi-planning coordination, optimizing the new pattern of urban and rural integration, and refining the construction and management measures adapted to the rural characteristics of developed areas.