Abstract: Systematically sorting out the correlation between landscape pattern and ecosystem service value (ESV) in traditional villages in the landscape is a key part of promoting rural tourism development and rural ecological revitalization. This paper takes Jiuxian Village, a traditional village of Li River Scenic Area, Guangxi Zhuang Autonomous Region, as an example. Based on the remote sensing images of 2005, 2010, 2015 and 2020, the landscape pattern index is selected, and the change of landscape pattern and ecosystem service value from 2005 to 2020 in Jiuxian Village is studied, and correlation analysis is conducted on the basis of the revised ecosystem service value equivalent table per unit area. The results show that:The landscape pattern of Jiuxian Village changes significantly from 2005 to 2020, including an increase in the area of construction land and river water surface, and a decrease in the area of forest land, cultivated land, grassland and unused land. Under the influence of tourism development, construction land is expanding and scattering, making the landscape pattern of the study area develop towards fragmentation and heterogeneity. The ESV of Jiuxian Village as a whole has been increasing year by year, and regulating services are the main ecosystem service function in the region, and the value of each ESV in the region is ranked as regulating services > supporting services > supplying services > cultural services. The landscape pattern index is significantly correlated with ESV, and each index has a different degree of influence on ESV. Therefore, reasonable adjustment of key landscape pattern characteristics can improve the effect of regional ecosystem services.