Abstract: Biodiversity conservation has become a national strategy, and has been incorporated into the long-term planning of each region. The practice of biodiversity conservation planning is considered an important initiative to maintain ecological balance and promote sustainable development, however, the working path of planning is still in the exploration stage. How to build an ecological network based on biodiversity conservation and implement it into spatial planning is currently a hot research issue. Based on the development history and working path of biodiversity conservation, the article takes the spatial planning practice of biodiversity conservation in Tongcheng County as an example, selects egrets and mandarin ducks as indicator species from actual species conservation, and discusses the working path of ecological network construction based on actual species, in order to provide reference for the construction of biodiversity conservation and ecological corridor system in the county.