Abstract: The transition of traditional e-commerce to live streaming commerce is propelled by shifts in consumer demand and the maturation of digital technologies. Current research predominantly examines the macro and meso-level spatial characteristics during the formation phase of traditional e-commerce villages, with relatively few detailed investigations incorporating micro-level architectural spaces during the live streaming transformation stage. Therefore, this study, leveraging POI data, interview insights, and literature review, takes Dayuan Village in Baiyun District, Guangzhou City, Guangdong Province as a case study to delve into the driving mechanisms of industrial development transformation on industrial spatial evolution within the e-commerce context. It also outlines the distinctive features of e-commerce industrial spatial evolution at both the village and individual building scales. The findings indicate that: 1) Industrial factors, such as supply chain structure and business models, significantly influence the functional elements, organizational patterns, and structural characteristics of industrial spaces, whereas regional factors like policy environments and operational conditions impart unique personalized traits. 2) The village-level industrial spatial structure, with Dayuan Road as its central development axis, has evolved from a 'scattered large-scale, small clustered point-like grouping' pattern to a 'core concentrated, periphery dispersed blocklike park' pattern. Concurrently, the industrial spatial structure of individual buildings has shifted from 'spontaneous functional mixing' to 'structured functional compounding'. 3) The industrial space in Dayuan Village has experienced a transition from a 'survival-oriented spatial paradigm' to a 'development-oriented spatial paradigm'. This research offers theoretical insights and practical guidance for the construction and optimization of industrial spaces in traditional e-commerce villages undergoing live streaming transformation.