Abstract: In the process of rural development, the spatial morphology of traditional villages has suffered varying degrees of damage, making the shaping, protection, and restoration of their distinctive spatial forms a critical issue in rural revitalization, particularly in the context of cultural revitalization recently. The spatial morphologies of ethnic traditional villages, however, retainintact relatively with distinctive ethnic characteristics. Introducing the concept of 'spatial genes', this study integrates perspectives from cultural geography and urban-rural planning. Taking Leishan County in Qiandongnan Prefecture, Guizhou Province as a case study, we selected 18 Miao traditional villages representative of the local ethnic characteristics. A spatial gene identification system for Miao traditional villages was constructed across five dimensions: spatial pattern, boundary morphology, street-network texture, building arrangement, and public spaces. Using software such as ArcGIS and CAD, combining with quantitative calculations, typological induction, and morphological pattern extraction, we conducted a multi-dimensional analysis of the spatial morphology characteristics of Miao traditional villages in Qiandongnan. The spatial genes were ultimately extracted and represented in the form of the spatial genes maps, with their features and underlying causes systematically analyzed. This research provides valuable insights for the preservation and inheritance of traditional villages, particularly those of ethnic traditional villages, and their unique spatial morphologies.